ABSTRACT
OBJECTIVE@#To establish a high-performance liquid chromatographic method (HPLC) for the simultaneous determination of 16 compounds from Artemisia ordosica.@*METHODS@#HPLC was used to analyze 16 quality indicators of A. ordosica. The HPLC conditions were as follows: Agilent Eclipse Plus C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile (A)-water (B) as mobile phase, gradient elution: 0-10 min, 75%-65% B; 10-30 min, 65%-35% B; and finally 30-40 min, 35%-15% B. The flow rate was 1.0 mL/min, the column temperature was 40 °C, the injection volume was 10 μL, and monitored by absorbance at 285 nm for compounds 1- 10, 12 and 225 nm for compounds 11, 13- 16.@*RESULTS@#Under the selected experimental chromatographic conditions, compounds 1- 16 showed good linearity (r > 0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%, 97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33% and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%, 1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively.@*CONCLUSION@#Based on the results of the HPLC analysis, it was concluded that p-hydroxycinnamic acid ( 1), O-hydroxycinnamic acid ( 2), coniferyl alcohol ( 5), 5,4'-dihydroxy-7,3'-dimethoxyflavanone ( 8), 5,4'-dihydroxy-7-methoxyflavanone ( 9), 5-hydroxy-7,4'-dimethoxyflavanone ( 12), dehydrofalcarindiol ( 13), arteordoyn A ( 14), dehydrofalcarinol ( 15) and capillarin ( 16) are best suited for the role of quality indicators of A. ordosica grown in different ecological environments.
ABSTRACT
OBJECTIVE: To establish the TLC identification and HPLC quantitative analysis method of Artemisia ordosica. METHODS: TLC and HPLC were used for qualitative and quantitative analysis of A. ordosica collected from five different regions. The TLC conditions were as follows: the reference substance was spathulenol, the adsorbent was silica gel G, the developing agent was petroleum ether (60-90 ℃)-acetone (5∶1) and the chromogenic color reagent was alcoholic solution of sulfuric acid (10%). The reference substance was 5,4′-dihydroxy-7-methoxyflavanone, the adsorbent was silica gel G, the developing agent was dichloromethane-ethyl acetate-formic acid (15∶1∶0.1) and the chromogenic reagent was ultraviolet lamp. The HPLC separation was set at performed on Topsil C18 (4.6 mm×250 mm,5 μm); the mobile phase was composed of water (A) and acetonitrile (B) and the gradient elution program was as follows: 0-15 min,25%-38% B;15-40 min,38%-45% B. The detection wavelength was 275 nm with column temperature kept at 30 ℃. RESULTS: The spots of reference substances (spathulenol and 5,4′-dihydroxy-7-methoxyflavanone) and A. ordosica in TLC had good repeatability and were easy to be identified. Under the HPLC conditions adopted in this study, all calibration curves exhibited good linearity (r>0.999 3). The recoveries of the method were 97.75%, 96.00%, 98.20%, 97.00%, 95.50%, 99.33%, 97.50%, 96.50%, and 97.33%, respectively. The RSDs were less than 2.0%. Compounds 1, 2, 5 and 8 were not detected in some samples, while compounds 3, 4, 6, 7 and 9 were detected and their content changes in different samples were (0.998±0.013)-(1.263±0.018), (0.108±0.002)-(0.301±0.005), (1.201±0.018)-(1.457±0.023), (0.635±0.011)-(0.801±0.013), (1.150±0.018)-(1.222±0.023) mg•g-1, respectively. CONCLUSION: The TLC identification and HPLC quantitative analysis of A. ordosica are established and can be used for the quality control of A. ordosica.
ABSTRACT
OBJECTIVE:To screen the agonist active ingredients of peroxisome proliferator-activated receptor-γ (PPAR-γ) in flavonoids from Artemisia ordosica,and provide reference for finding antidiabetic agents in A.ordosica.METHODS:Using known PPAR-γagonist rosiglitazone as positive control,molecular docking technology was conducted for docking one by one for 18 flavonoids and PPAR-7 targets obtained from A.ordosica.It was compared with binding affinities and binding modes of compounds and PPAR-7 targets,and the possible PPAR-γ agonist ingredients in A.ordosica were screened.RESULTS:5 flavonoids showed good docking affinities,in which,compound 3 (5,3',4'-trihydroxy-7-methoxyflavone) showed the highest (-8.3 kcal/mol).Docking mode analysis showed that the phenol oxygen on ring A and ring B of the flavonoids with LBD active site of PPAR-γ formed one (Tyr327) or two hydrogen bonding (Tyr327,Arg288),which played an important role in the binding of flavonoids and PPAR-γ and the stability of PPAR-γ conformation.CONCLUSIONS:Results of virtual screening in molecular docking technology indicate that flavonoids (mostly containing multiple free phenolic hydroxyl groups) in can easily form good docking mode and high affinity with PPAR-γ,showing potential antidiabetic activity.The study can provide reference for further research of chemical ingredients for the treatment of type 2 diabetes.